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Cleaved-Caspase-4 p20 (Q81) rabbit pAb

ES1019

規(guī)格：	價格：
50μL	￥1280.00
100μL	￥1980.00

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Overview

Product name:	Cleaved-Caspase-4 p20 (Q81) rabbit pAb
Reactivity:	Human
Alternative Names:	CASP4; ICH2; Caspase-4; CASP-4; ICE(rel)-II; Protease ICH-2; Protease TX
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human Caspase 4. AA range:62-111
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	43+22kD
GeneID:	837
Human Swiss-Prot No:	P49662
Cellular localization:	Cytoplasm, cytosol . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion . Inflammasome . Secreted . Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630). .
Background:	This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody diluted at 1:2000
Western Blot analysis of 3T3 cells using Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from Jurkat cells, using Caspase 4 (p20,Cleaved-Gln81) Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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