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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 2.201 | 2.114 |
10.00 | 1.612 | 1.525 |
5.00 | 1.273 | 1.186 |
2.50 | 0.871 | 0.784 |
1.25 | 0.567 | 0.480 |
0.63 | 0.323 | 0.236 |
0.32 | 0.258 | 0.171 |
0.00 | 0.087 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-99% | 92% |
EDTA plasma(n=5) | 92-106% | 99% |
Heparin plasma(n=5) | 81-97% | 89% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-92% | 86-97% | 90-101% | 92-102% |
EDTA plasma(n=5) | 85-97% | 95-107% | 85-94% | 82-96% |
Heparin plasma(n=5) | 83-89% | 88-104% | 97-103% | 89-101% |
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