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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 2.179 | 2.090 |
5.00 | 1.675 | 1.586 |
2.50 | 1.162 | 1.073 |
1.25 | 0.896 | 0.807 |
0.63 | 0.517 | 0.428 |
0.32 | 0.377 | 0.288 |
0.16 | 0.236 | 0.147 |
0.00 | 0.089 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 86-99% | 92% |
EDTA plasma(n=5) | 80-95% | 87% |
Heparin plasma(n=5) | 91-105% | 98% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 89-97% | 85-97% | 87-96% | 88-97% |
EDTA plasma(n=5) | 91-97% | 81-93% | 89-103% | 82-91% |
Heparin plasma(n=5) | 83-99% | 98-105% | 96-101% | 88-95% |
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