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日期：2023-08-06
IF：5.6標(biāo)準(zhǔn)曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 2.096 | 2.009 |
| 5.00 | 1.686 | 1.599 |
| 2.50 | 1.120 | 1.033 |
| 1.25 | 0.828 | 0.741 |
| 0.63 | 0.567 | 0.480 |
| 0.32 | 0.318 | 0.231 |
| 0.16 | 0.248 | 0.161 |
| 0.00 | 0.087 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 88-102% | 95% |
| EDTA plasma(n=5) | 82-95% | 88% |
| Heparin plasma(n=5) | 79-93% | 86% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 81-93% | 87-101% | 96-105% | 96-105% |
| EDTA plasma(n=5) | 88-96% | 87-98% | 93-101% | 91-99% |
| Heparin plasma(n=5) | 85-97% | 93-101% | 87-98% | 79-96% |
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