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日期：2023-06-01
IF：6.78標(biāo)準(zhǔn)曲線
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 2000.00 | 2.054 | 1.959 |
| 1000.00 | 1.527 | 1.432 |
| 500.00 | 1.251 | 1.156 |
| 250.00 | 0.834 | 0.739 |
| 125.00 | 0.453 | 0.358 |
| 62.50 | 0.326 | 0.231 |
| 31.25 | 0.187 | 0.092 |
| 0.00 | 0.095 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant NFκB-p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NFκB-p65 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-107% | 98% |
| EDTA plasma(n=5) | 93-107% | 100% |
| Heparin plasma(n=5) | 85-97% | 91% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NFκB-p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 86-94% | 81-95% | 88-97% | 85-92% |
| EDTA plasma(n=5) | 83-96% | 93-102% | 86-94% | 90-98% |
| Heparin plasma(n=5) | 92-106% | 85-97% | 96-105% | 88-96% |
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