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下載說明 ①Overview 文獻
日期：2023-12-15
IF：7.9標準曲線
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 20000.00 | 0.199 | |
| 10000.00 | 0.397 | |
| 5000.00 | 0.612 | |
| 2500.00 | 0.895 | |
| 1250.00 | 1.236 | |
| 625.00 | 1.585 | |
| 312.50 | 1.775 | |
| 0.00 | 2.028 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant 17-OHP and the recovery rates were calculated by comparing the measured value to the expected amount of 17-OHP in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 96-107% | 101% |
| Heparin plasma(n=5) | 90-105% | 89% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 17-OHP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 82-94% | 85-96% | 82-96% | 95-102% |
| EDTA plasma(n=5) | 86-97% | 90-98% | 80-93% | 92-105% |
| Heparin plasma(n=5) | 86-99% | 93-106% | 89-97% | 85-94% |
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