• <th id="abimu"></th>

      1. 產(chǎn)品中心

        大鼠皮質(zhì)酮(Cort)酶聯(lián)免疫吸附檢測(cè)試劑盒
        ELK8633
        規(guī)格: 價(jià)格:
        48T ¥1680.00
        96T ¥2400.00

        Overview 文獻(xiàn)

        Product name: Rat Cort(Corticosterone) ELISA Kit
        Reactivity: Rat
        Alternative Names: Corticosrone
        Assay Type: Competitive Inhibition
        Sensitivity: 0.51 ng/mL
        Standard: 100 ng/mL
        Detection Range: 1.57-100 ng/mL
        Sample Type: serum, plasma, urine and other biological fluids
        Assay Length: 2h
        Research Area: Infection immunity;Endocrinology;Hormone metabolism;
        Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Cort protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Cort. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

        標(biāo)準(zhǔn)曲線

        Concentration (ng/mL) OD Corrected OD
        100.00 0.197
        50.00 0.275
        25.00 0.583
        12.50 0.895
        6.25 1.156
        3.13 1.583
        1.57 1.762
        0.00 2.101

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant Cort and the recovery rates were calculated by comparing the measured value to the expected amount of Cort in samples.
        Matrix Recovery range Average
        serum(n=5) 96-107% 101%
        EDTA plasma(n=5) 87-99% 92%
        Heparin plasma(n=5) 84-96% 98%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cort and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 85-97% 90-99% 87-103% 85-101%
        EDTA plasma(n=5) 85-97% 93-106% 83-95% 88-103%
        Heparin plasma(n=5) 87-95% 85-96% 89-100% 85-96%
        關(guān)閉

        在線咨詢

        Online consultation

        • 在線咨詢
        • 技術(shù)支持

        關(guān)注微信公眾號(hào)

        微信掃一掃立即咨詢

        微信掃一掃立即咨詢

        關(guān)閉
        一级a爱片国产亚洲精品,97se亚洲综合色区,日朝欧美亚洲精品,欧洲韩国亚洲一区二区三区

      2. <th id="abimu"></th>