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人8-羥基脫氧鳥(niǎo)苷(8-OHdG)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK8533

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Human 8-OHdG(DNA Damage) ELISA Kit
Reactivity:	Human
Alternative Names:	8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 8-Hydroxydeoxyguanosine
Assay Type:	Competitive Inhibition
Sensitivity:	26.29 pg/mL
Standard:	6000 pg/mL
Detection Range:	93.75-6000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

Toxics

Biomonitoring of Oxidative-Stress-Related Genotoxic Damage in Patients with End-Stage Renal Disease

日期：2024-01-12 IF：4.6

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
6000.00	0.191
3000.00	0.367
1500.00	0.596
750.00	0.861
375.00	1.157
187.50	1.585
93.75	1.775
0.00	2.123

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	80-93%	86%
Heparin plasma(n=5)	94-105%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.