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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
200.00 | 2.146 | 2.080 |
100.00 | 1.563 | 1.497 |
50.00 | 1.088 | 1.022 |
25.00 | 0.841 | 0.775 |
12.50 | 0.553 | 0.487 |
6.25 | 0.315 | 0.249 |
3.13 | 0.206 | 0.140 |
0.00 | 0.066 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 95-104% | 98% |
EDTA plasma(n=5) | 82-90% | 86% |
Heparin plasma(n=5) | 84-92% | 87% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 82-93% | 96-105% | 87-98% | 85-97% |
EDTA plasma(n=5) | 92-106% | 92-101% | 80-95% | 87-96% |
Heparin plasma(n=5) | 85-96% | 87-101% | 89-102% | 85-98% |
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