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        小鼠雌激素(Estrogen)酶聯(lián)免疫吸附檢測(cè)試劑盒
        ELK8407
        規(guī)格: 價(jià)格:
        48T ¥2240.00
        96T ¥3200.00

        Overview 文獻(xiàn)

        Product name: Mouse Estrogen ELISA Kit
        Reactivity: Mouse
        Alternative Names: E ; Estrogen
        Assay Type: Competitive Inhibition
        Sensitivity: 4.15 pg/mL
        Standard: 1000 pg/mL
        Detection Range: 15.625-1000 pg/mL
        Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
        Assay Length: 2h
        Research Area: Reproductive science;
        Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Estrogen protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.

        標(biāo)準(zhǔn)曲線

        Concentration (pg/mL) OD Corrected OD
        1000.00 0.215
        500.00 0.352
        250.00 0.596
        125.00 0.861
        62.50 1.157
        31.25 1.585
        15.63 1.775
        0.00 2.115

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant Estrogen and the recovery rates were calculated by comparing the measured value to the expected amount of Estrogen in samples.
        Matrix Recovery range Average
        serum(n=5) 80-95% 87%
        EDTA plasma(n=5) 86-99% 92%
        Heparin plasma(n=5) 82-97% 99%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estrogen and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 91-99% 88-97% 96-105% 95-103%
        EDTA plasma(n=5) 92-101% 87-98% 85-92% 79-96%
        Heparin plasma(n=5) 79-94% 92-101% 87-96% 82-90%
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