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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
200.00 | 0.199 | |
100.00 | 0.385 | |
50.00 | 0.621 | |
25.00 | 0.998 | |
12.50 | 1.253 | |
6.25 | 1.522 | |
3.13 | 1.786 | |
0.00 | 2.375 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 92-107% | 101% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 95-103% | 86-92% | 86-92% | 89-97% |
EDTA plasma(n=5) | 95-104% | 82-96% | 92-105% | 83-92% |
Heparin plasma(n=5) | 79-94% | 96-105% | 87-101% | 82-90% |
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