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        人殺菌肽LL-37(LL-37)酶聯(lián)免疫吸附檢測試劑盒
        
                    ELK8189
                    
        
                        
        
                            
        
                                規(guī)格：
                                價格：
                            
        
                                                        
        
                                48T
                                ￥2240.00
                            
        
                                                        
        
                                96T
                                ￥3200.00
                            
        
                                                    
        
                    
        
                    
                
        
            
        
        
        

                
        
            
        
                
        Overview
                    文獻
        
                
        
                    
        
                        
        
                            
        
                                
        
                                    
        
                                        Product name:
                                        Human LL-37(Antibacterial Protein LL-37) ELISA Kit
                                    
        
                                                                            
        
                                            Reactivity:
                                            Human
                                        
        
                                                                                
        
                                            Alternative Names:
                                            CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide
                                        
        
                                                                                
        
                                           Assay Type:
                                           Sandwich
                                           
                                        
        
                                                                                
        
                                           Sensitivity:
                                           0.27 ng/mL
                                           
                                        
        
                                                                                
        
                                           Standard:
                                           50 ng/mL
                                           
                                        
        
                                                                                
        
                                           Detection Range:
                                           0.79-50 ng/mL
                                           
                                        
        
                                                                                
        
                                           Sample Type:
                                           serum, plasma and other biological fluids
                                           
                                        
        
                                                                                
        
                                           Assay Length:
                                           3.5h
                                           
                                        
        
                                                                                
        
                                           Research Area:
                                           Infection immunity;
                                           
                                        
        
                                                                                
        
                                           Test principle:
                                           The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.
                                           
                                        
        
                                                                        
        
                            
        
                                                        
                                                    
        
                    
        
                
        
            
        
                        
        
                
        標準曲線
        
                
        
                    
        
                        
        
                            
        
                                Concentration (ng/mL)
                                OD
                                                                Corrected OD
                                                            
        
                                                        
        
                                50.00
                                2.054
                                                                1.971
                                                            
        
                                                        
        
                                25.00
                                1.587
                                                                1.504
                                                            
        
                                                        
        
                                12.50
                                1.027
                                                                0.944
                                                            
        
                                                        
        
                                6.25
                                0.866
                                                                0.783
                                                            
        
                                                        
        
                                3.13
                                0.509
                                                                0.426
                                                            
        
                                                        
        
                                1.57
                                0.335
                                                                0.252
                                                            
        
                                                        
        
                                0.79
                                0.228
                                                                0.145
                                                            
        
                                                        
        
                                0.00
                                0.083
                                                                0.000
                                                            
        
                                                    
        
                    
        

                    
        
                
        
            
        
                        
        
                
        精密度
        
                
        
                    
        Intra-assay Precision (Precision within an assay)：CV%<8%
        
                    
        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
        
                    
        Inter-assay Precision (Precision between assays)：CV%<10%
        
                    
        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
        
                
        
            
        
                        
        
                
        回收率
        
                
        
                    
        Matrices listed below were spiked with certain level of recombinant LL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of LL-37 in samples.
        
                    
        
                        
        
                            
        
                                Matrix
                                Recovery range
                                Average
                            
        
                            
        
                                serum(n=5)
                                82-94%
                                88%
                            
        
                            
        
                                EDTA plasma(n=5)
                                85-97%
                                91%
                            
        
                            
        
                                Heparin plasma(n=5)
                                82-94%
                                88%
                            
        
                        
        
                    
        
                
        
            
        
                        
        
                
        線性
        
                
        
                    
        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        
                    
        
                        
        
                            
        
                                Matrix
                                1:2
                                1:4
                                1:8
                                1:16
                            
        
                            
        
                                serum(n=5)
                                87-98%
                                83-94%
                                85-98%
                                85-97%
                            
        
                            
        
                                EDTA plasma(n=5)
                                98-105%
                                92-101%
                                85-105%
                                87-98%
                            
        
                            
        
                                Heparin plasma(n=5)
                                85-92%
                                79-96%
                                89-100%
                                85-96%
                            
        
                        
        
                    
        
                
        
            
        
                    
        
    
        
    




        
    
        
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