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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
50.00 | 2.054 | 1.971 |
25.00 | 1.587 | 1.504 |
12.50 | 1.027 | 0.944 |
6.25 | 0.866 | 0.783 |
3.13 | 0.509 | 0.426 |
1.57 | 0.335 | 0.252 |
0.79 | 0.228 | 0.145 |
0.00 | 0.083 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 82-94% | 88% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 82-94% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-98% | 83-94% | 85-98% | 85-97% |
EDTA plasma(n=5) | 98-105% | 92-101% | 85-105% | 87-98% |
Heparin plasma(n=5) | 85-92% | 79-96% | 89-100% | 85-96% |
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