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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 0.185 | |
500.00 | 0.389 | |
250.00 | 0.577 | |
125.00 | 0.861 | |
62.50 | 1.152 | |
31.25 | 1.522 | |
15.63 | 1.789 | |
0.00 | 2.115 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 87-93% | 90% |
EDTA plasma(n=5) | 80-90% | 85% |
Heparin plasma(n=5) | 84-106% | 87% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 83-95% | 88-102% | 85-96% | 90-99% |
EDTA plasma(n=5) | 85-97% | 93-101% | 97-103% | 91-99% |
Heparin plasma(n=5) | 81-97% | 85-96% | 78-90% | 91-99% |
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