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        S-腺苷蛋氨酸(SAM)酶聯(lián)免疫吸附檢測試劑盒
        ELK8108
        規(guī)格: 價格:
        48T ¥2240.00
        96T ¥3200.00

        Overview 文獻

        Product name: SAM(S-Adenosyl Methionine) ELISA Kit
        Reactivity: General
        Alternative Names: SAMe; SAM-e; S-Adenosylmethionine; S-(5'-Adenosyl)-L-Methionine Chloride
        Assay Type: Competitive Inhibition
        Sensitivity: 4.84 ng/mL
        Standard: 1000 ng/mL
        Detection Range: 15.63-1000 ng/mL
        Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
        Assay Length: 2h
        Research Area: Enzyme & Kinase;
        Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SAM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SAM in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (ng/mL) OD Corrected OD
        1000.00 0.185
        500.00 0.389
        250.00 0.577
        125.00 0.861
        62.50 1.152
        31.25 1.522
        15.63 1.789
        0.00 2.115

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant SAM and the recovery rates were calculated by comparing the measured value to the expected amount of SAM in samples.
        Matrix Recovery range Average
        serum(n=5) 87-93% 90%
        EDTA plasma(n=5) 80-90% 85%
        Heparin plasma(n=5) 84-106% 87%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 83-95% 88-102% 85-96% 90-99%
        EDTA plasma(n=5) 85-97% 93-101% 97-103% 91-99%
        Heparin plasma(n=5) 81-97% 85-96% 78-90% 91-99%
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