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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 2.082 | 1.994 |
5.00 | 1.841 | 1.753 |
2.50 | 1.231 | 1.143 |
1.25 | 0.799 | 0.711 |
0.63 | 0.438 | 0.350 |
0.32 | 0.387 | 0.299 |
0.16 | 0.225 | 0.137 |
0.00 | 0.088 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 83-99% | 95% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 85-97% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-97% | 90-99% | 85-97% | 79-101% |
EDTA plasma(n=5) | 86-97% | 93-102% | 87-98% | 83-97% |
Heparin plasma(n=5) | 95-104% | 82-96% | 92-103% | 90-99% |
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