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下載說明 ①Overview 文獻
日期：2021-10-20
IF：3.211標(biāo)準(zhǔn)曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 20.00 | 2.379 | 2.296 |
| 10.00 | 1.621 | 1.538 |
| 5.00 | 1.115 | 1.032 |
| 2.50 | 0.735 | 0.652 |
| 1.25 | 0.555 | 0.472 |
| 0.63 | 0.298 | 0.215 |
| 0.32 | 0.223 | 0.140 |
| 0.00 | 0.083 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant NF-κBp105 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κBp105 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 81-93% | 87% |
| Heparin plasma(n=5) | 82-94% | 88% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κBp105 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-102% | 82-91% | 86-93% | 81-94% |
| EDTA plasma(n=5) | 86-94% | 85-95% | 89-99% | 85-95% |
| Heparin plasma(n=5) | 89-97% | 85-94% | 79-96% | 85-99% |
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