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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.185 | 2.096 |
500.00 | 1.562 | 1.473 |
250.00 | 1.217 | 1.128 |
125.00 | 0.823 | 0.734 |
62.50 | 0.513 | 0.424 |
31.25 | 0.336 | 0.247 |
15.63 | 0.248 | 0.159 |
0.00 | 0.089 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 84-102% | 93% |
Heparin plasma(n=5) | 87-95% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 81-93% | 86-98% | 91-103% | 82-95% |
EDTA plasma(n=5) | 85-92% | 82-96% | 87-98% | 93-101% |
Heparin plasma(n=5) | 88-102% | 86-92% | 91-103% | 89-97% |
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