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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 2.121 | 2.039 |
5.00 | 1.674 | 1.592 |
2.50 | 1.114 | 1.032 |
1.25 | 0.812 | 0.730 |
0.63 | 0.623 | 0.541 |
0.32 | 0.377 | 0.295 |
0.16 | 0.252 | 0.170 |
0.00 | 0.082 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 83-98% | 90% |
EDTA plasma(n=5) | 81-95% | 88% |
Heparin plasma(n=5) | 88-102% | 95% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-94% | 87-101% | 89-99% | 85-95% |
EDTA plasma(n=5) | 95-104% | 82-94% | 86-96% | 81-97% |
Heparin plasma(n=5) | 86-95% | 91-106% | 87-100% | 95-101% |
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