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小鼠b2微球蛋白(BMG/b2-MG)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK5100

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Mouse BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
Reactivity:	Mouse
Alternative Names:	Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Type:	Sandwich
Sensitivity:	0.16 ng/mL
Standard:	300 ng/mL
Detection Range:	4.69-300 ng/mL
Sample Type:	serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;Kidney biomarker;
Uniprot ID:	P01887
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

acta pharmacologica sinica

G protein-coupled receptor kinase 2 as a novel therapeutic target for gland fibrosis of Sj?gren’s syndrome

日期：2024-07-25 IF：6.9

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
300.00	2.119	2.046
150.00	1.663	1.590
75.00	1.267	1.194
37.50	0.874	0.801
18.75	0.528	0.455
9.38	0.362	0.289
4.69	0.232	0.159
0.00	0.073	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant BMG/b2-MG and the recovery rates were calculated by comparing the measured value to the expected amount of BMG/b2-MG in samples.

Matrix	Recovery range	Average
serum(n=5)	80-96%	88%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	83-99%	95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMG/b2-MG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.