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小鼠趨化因子C-C-基元受體7(CCR7)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK3661

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Mouse CCR7(Chemokine C-C-Motif Receptor 7) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CD197; CCR7; BLR2; CDw197; CMKBR7; EBI1; Epstein-Barr Virus Induced Protein 1; EBV-induced G-protein coupled receptor 1; MIP-3 beta receptor
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Uniprot ID:	P47774
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CCR7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CCR7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CCR7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CCR7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Dermatopathology

The Effect of Bacillus coagulans Induced Interactions among Intestinal Bacteria, Metabolites, and Inflammatory Molecules in Improving Natural Skin Aging

日期：2023-09-18 IF：1.9

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.266	2.185
5.00	1.527	1.446
2.50	1.144	1.063
1.25	0.901	0.820
0.63	0.556	0.475
0.32	0.335	0.254
0.16	0.235	0.154
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CCR7 and the recovery rates were calculated by comparing the measured value to the expected amount of CCR7 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-98%	90%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	86-99%	92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCR7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.