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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 1.932 | 1.851 |
250.00 | 1.602 | 1.521 |
125.00 | 1.185 | 1.104 |
62.50 | 0.896 | 0.815 |
31.25 | 0.539 | 0.458 |
15.63 | 0.239 | 0.158 |
7.82 | 0.208 | 0.127 |
0.00 | 0.081 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 86-99% | 92% |
EDTA plasma(n=5) | 80-95% | 87% |
Heparin plasma(n=5) | 80-95% | 87% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 79-96% | 89-101% | 85-97% |
EDTA plasma(n=5) | 86-100% | 93-108% | 80-101% | 87-96% |
Heparin plasma(n=5) | 96-105% | 95-103% | 95-103% | 87-98% |
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