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        大鼠對氧磷酶1(PON1)酶聯(lián)免疫吸附檢測試劑盒
        
                    ELK2984
                    
        
                        
        
                            
        
                                規(guī)格：
                                價格：
                            
        
                                                        
        
                                48T
                                ￥1960.00
                            
        
                                                        
        
                                96T
                                ￥2800.00
                            
        
                                                    
        
                    
        
                    
                
        
            
        
        
        

                
        
            
        
                
        Overview
                    文獻
        
                
        
                    
        
                        
        
                            
        
                                
        
                                    
        
                                        Product name:
                                        Rat PON1(Paraoxonase 1) ELISA Kit
                                    
        
                                                                            
        
                                            Reactivity:
                                            Rat
                                        
        
                                                                                
        
                                            Alternative Names:
                                            ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
                                        
        
                                                                                
        
                                           Assay Type:
                                           Sandwich
                                           
                                        
        
                                                                                
        
                                           Sensitivity:
                                           0.066 ng/mL
                                           
                                        
        
                                                                                
        
                                           Standard:
                                           10 ng/mL
                                           
                                        
        
                                                                                
        
                                           Detection Range:
                                           0.16-10 ng/mL
                                           
                                        
        
                                                                                
        
                                           Sample Type:
                                           serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
                                           
                                        
        
                                                                                
        
                                           Assay Length:
                                           3.5h
                                           
                                        
        
                                                                                
        
                                           Research Area:
                                           Metabolic pathway;Endocrinology;
                                           
                                        
        
                                                                                
        
                                           Uniprot ID:
                                           P55159
                                           
                                        
        
                                                                                
        
                                           Test principle:
                                           The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PON1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PON1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PON1 in the samples is then determined by comparing the OD of the samples to the standard curve.
                                           
                                        
        
                                                                        
        
                            
        
                                                        
                                                    
        
                    
        
                
        
            
        
                        
        
                
        標準曲線
        
                
        
                    
        
                        
        
                            
        
                                Concentration (ng/mL)
                                OD
                                                                Corrected OD
                                                            
        
                                                        
        
                                10.00
                                2.075
                                                                1.994
                                                            
        
                                                        
        
                                5.00
                                1.593
                                                                1.512
                                                            
        
                                                        
        
                                2.50
                                1.175
                                                                1.094
                                                            
        
                                                        
        
                                1.25
                                0.971
                                                                0.890
                                                            
        
                                                        
        
                                0.63
                                0.582
                                                                0.501
                                                            
        
                                                        
        
                                0.32
                                0.339
                                                                0.258
                                                            
        
                                                        
        
                                0.16
                                0.241
                                                                0.160
                                                            
        
                                                        
        
                                0.00
                                0.081
                                                                0.000
                                                            
        
                                                    
        
                    
        

                    
        
                
        
            
        
                        
        
                
        精密度
        
                
        
                    
        Intra-assay Precision (Precision within an assay)：CV%<8%
        
                    
        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
        
                    
        Inter-assay Precision (Precision between assays)：CV%<10%
        
                    
        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
        
                
        
            
        
                    
        
    
        
    




        
    
        
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