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大鼠半乳糖凝集素3(GAL3)酶聯(lián)免疫吸附檢測試劑盒

ELK2806

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Rat GAL3(Galectin 3) ELISA Kit
Reactivity:	Rat
Alternative Names:	LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29
Assay Type:	Sandwich
Sensitivity:	0.055 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;
Uniprot ID:	P08699
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GAL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GAL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GAL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GAL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Toxicology and Environmental Health Sciences

Anti-inflammatory and antioxidative effects of dexpanthenol on nicotine-induced lung injury in rats

日期：2023-08-09 IF：1.2

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.121	2.034
5.00	1.763	1.676
2.50	1.284	1.197
1.25	0.847	0.760
0.63	0.639	0.552
0.32	0.481	0.394
0.16	0.198	0.111
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GAL3 and the recovery rates were calculated by comparing the measured value to the expected amount of GAL3 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	86-99%	92%
Heparin plasma(n=5)	90-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAL3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.