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        人血小板衍生生長(zhǎng)因子受體β(PDGFRb)酶聯(lián)免疫吸附檢測(cè)試劑盒
        
                    ELK2769
                    
        
                        
        
                            
        
                                規(guī)格：
                                價(jià)格：
                            
        
                                                        
        
                                48T
                                ￥1960.00
                            
        
                                                        
        
                                96T
                                ￥2800.00
                            
        
                                                    
        
                    
        
                    
                
        
            
        
        
        

                
        
            
        
                
        Overview
                    文獻(xiàn)
        
                
        
                    
        
                        
        
                            
        
                                
        
                                    
        
                                        Product name:
                                        Human PDGFRb(Platelet Derived Growth Factor Receptor Beta) ELISA Kit
                                    
        
                                                                            
        
                                            Reactivity:
                                            Human
                                        
        
                                                                                
        
                                            Alternative Names:
                                            CD140b; JTK12; PDGF-R-Beta; PDGFR; PDGFR1; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; Beta-type platelet-derived growth factor receptor
                                        
        
                                                                                
        
                                           Assay Type:
                                           Sandwich
                                           
                                        
        
                                                                                
        
                                           Sensitivity:
                                           0.054 ng/mL
                                           
                                        
        
                                                                                
        
                                           Standard:
                                           10 ng/mL
                                           
                                        
        
                                                                                
        
                                           Detection Range:
                                           0.16-10 ng/mL
                                           
                                        
        
                                                                                
        
                                           Sample Type:
                                           tissue homogenates, cell lysates and other biological fluids
                                           
                                        
        
                                                                                
        
                                           Assay Length:
                                           3.5h
                                           
                                        
        
                                                                                
        
                                           Research Area:
                                           Signal transduction;Tumor immunity;Infection immunity;Hematology;
                                           
                                        
        
                                                                                
        
                                           Uniprot ID:
                                           P09619
                                           
                                        
        
                                                                                
        
                                           Test principle:
                                           The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDGFRb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDGFRb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDGFRb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDGFRb in the samples is then determined by comparing the OD of the samples to the standard curve.
                                           
                                        
        
                                                                        
        
                            
        
                                                        
                                                    
        
                    
        
                
        
            
        
                        
        
                
        標(biāo)準(zhǔn)曲線
        
                
        
                    
        
                        
        
                            
        
                                Concentration (ng/mL)
                                OD
                                                                Corrected OD
                                                            
        
                                                        
        
                                10.00
                                1.933
                                                                1.835
                                                            
        
                                                        
        
                                5.00
                                1.582
                                                                1.484
                                                            
        
                                                        
        
                                2.50
                                1.217
                                                                1.119
                                                            
        
                                                        
        
                                1.25
                                1.005
                                                                0.907
                                                            
        
                                                        
        
                                0.63
                                0.617
                                                                0.519
                                                            
        
                                                        
        
                                0.32
                                0.324
                                                                0.226
                                                            
        
                                                        
        
                                0.16
                                0.202
                                                                0.104
                                                            
        
                                                        
        
                                0.00
                                0.098
                                                                0.000
                                                            
        
                                                    
        
                    
        

                    
        
                
        
            
        
                        
        
                
        精密度
        
                
        
                    
        Intra-assay Precision (Precision within an assay)：CV%<8%
        
                    
        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
        
                    
        Inter-assay Precision (Precision between assays)：CV%<10%
        
                    
        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
        
                
        
            
        
                    
        
    
        
    




        
    
        
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