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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 2.058 | 1.970 |
10.00 | 1.472 | 1.384 |
5.00 | 1.197 | 1.109 |
2.50 | 0.921 | 0.833 |
1.25 | 0.597 | 0.509 |
0.63 | 0.300 | 0.212 |
0.32 | 0.216 | 0.128 |
0.00 | 0.088 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 92-105% | 98% |
Heparin plasma(n=5) | 84-96% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-102% | 87-102% | 85-96% | 87-101% |
EDTA plasma(n=5) | 86-96% | 85-97% | 89-101% | 85-97% |
Heparin plasma(n=5) | 85-94% | 87-101% | 86-97% | 88-95% |
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