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下載說明 ①Overview 文獻
日期：2024-07-09
IF：3.4標準曲線
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 1.989 | 1.900 |
| 500.00 | 1.633 | 1.544 |
| 250.00 | 1.121 | 1.032 |
| 125.00 | 0.726 | 0.637 |
| 62.50 | 0.529 | 0.440 |
| 31.25 | 0.322 | 0.233 |
| 15.63 | 0.252 | 0.163 |
| 0.00 | 0.089 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant I-PTH and the recovery rates were calculated by comparing the measured value to the expected amount of I-PTH in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 86-99% | 92% |
| EDTA plasma(n=5) | 80-95% | 87% |
| Heparin plasma(n=5) | 83-95% | 89% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of I-PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 95-102% | 87-101% | 82-95% | 81-93% |
| EDTA plasma(n=5) | 85-92% | 79-96% | 95-102% | 87-98% |
| Heparin plasma(n=5) | 89-105% | 81-94% | 96-105% | 92-103% |
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