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        人全段甲狀旁腺素(I-PTH)酶聯(lián)免疫吸附檢測試劑盒
        ELK2427
        規(guī)格: 價格:
        48T ¥1960.00
        96T ¥2800.00

        Overview 文獻

        Product name: Human I-PTH(IntactParathormone) ELISA Kit
        Reactivity: Human
        Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
        Assay Type: Sandwich
        Sensitivity: 4.95 pg/mL
        Standard: 1000 pg/mL
        Detection Range: 15.63-1000 pg/mL
        Sample Type: serum, plasma and other biological fluids
        Assay Length: 3.5h
        Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (pg/mL) OD Corrected OD
        1000.00 1.989 1.900
        500.00 1.633 1.544
        250.00 1.121 1.032
        125.00 0.726 0.637
        62.50 0.529 0.440
        31.25 0.322 0.233
        15.63 0.252 0.163
        0.00 0.089 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant I-PTH and the recovery rates were calculated by comparing the measured value to the expected amount of I-PTH in samples.
        Matrix Recovery range Average
        serum(n=5) 86-99% 92%
        EDTA plasma(n=5) 80-95% 87%
        Heparin plasma(n=5) 83-95% 89%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of I-PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 95-102% 87-101% 82-95% 81-93%
        EDTA plasma(n=5) 85-92% 79-96% 95-102% 87-98%
        Heparin plasma(n=5) 89-105% 81-94% 96-105% 92-103%
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