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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 0.189 | |
500.00 | 0.357 | |
250.00 | 0.575 | |
125.00 | 0.878 | |
62.50 | 1.169 | |
31.25 | 1.537 | |
15.63 | 1.793 | |
0.00 | 2.226 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 90-105% | 97% |
EDTA plasma(n=5) | 88-102% | 95% |
Heparin plasma(n=5) | 80-92% | 86% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-101% | 79-96% | 90-98% | 92-101% |
EDTA plasma(n=5) | 85-92% | 89-96% | 79-98% | 81-92% |
Heparin plasma(n=5) | 88-95% | 82-94% | 85-96% | 78-90% |
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