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Concentration (mIU/mL) | OD | Corrected OD |
---|---|---|
30.00 | 2.258 | 2.185 |
15.00 | 1.686 | 1.613 |
7.50 | 1.185 | 1.112 |
3.75 | 0.806 | 0.733 |
1.88 | 0.585 | 0.512 |
0.94 | 0.322 | 0.249 |
0.47 | 0.258 | 0.185 |
0.00 | 0.073 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 87-99% | 93% |
EDTA plasma(n=5) | 78-92% | 85% |
Heparin plasma(n=5) | 85-99% | 92% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-94% | 92-101% | 84-92% | 78-90% |
EDTA plasma(n=5) | 91-98% | 83-101% | 87-98% | 95-102% |
Heparin plasma(n=5) | 85-96% | 85-101% | 84-98% | 85-93% |
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