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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2000.00 | 1.963 | 1.878 |
1000.00 | 1.611 | 1.526 |
500.00 | 1.067 | 0.982 |
250.00 | 0.782 | 0.697 |
125.00 | 0.548 | 0.463 |
62.50 | 0.365 | 0.280 |
31.25 | 0.187 | 0.102 |
0.00 | 0.085 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-97% | 91% |
EDTA plasma(n=5) | 83-99% | 95% |
Heparin plasma(n=5) | 85-97% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 92-104% | 82-96% | 95-102% | 87-98% |
EDTA plasma(n=5) | 78-92% | 79-93% | 94-106% | 83-97% |
Heparin plasma(n=5) | 80-92% | 86-102% | 87-103% | 85-101% |
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