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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
8000.00 | 1.941 | 1.853 |
4000.00 | 1.484 | 1.396 |
2000.00 | 1.036 | 0.948 |
1000.00 | 0.625 | 0.537 |
500.00 | 0.373 | 0.285 |
250.00 | 0.300 | 0.212 |
125.00 | 0.216 | 0.128 |
0.00 | 0.088 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 91-99% | 95% |
EDTA plasma(n=5) | 82-95% | 88% |
Heparin plasma(n=5) | 83-98% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-97% | 90-98% | 80-93% | 92-105% |
EDTA plasma(n=5) | 87-98% | 85-92% | 79-96% | 89-100% |
Heparin plasma(n=5) | 79-91% | 89-103% | 82-95% | 86-99% |
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