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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.219 | 2.131 |
500.00 | 1.636 | 1.548 |
250.00 | 1.132 | 1.044 |
125.00 | 0.872 | 0.784 |
62.50 | 0.547 | 0.459 |
31.25 | 0.300 | 0.212 |
15.63 | 0.216 | 0.128 |
0.00 | 0.088 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 92-107% | 99% |
EDTA plasma(n=5) | 87-99% | 93% |
Heparin plasma(n=5) | 78-92% | 85% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-94% | 91-105% | 87-99% | 95-100% |
EDTA plasma(n=5) | 85-90% | 83-96% | 95-102% | 88-95% |
Heparin plasma(n=5) | 83-103% | 82-93% | 86-95% | 81-96% |
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