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        大鼠白介素19(IL19)酶聯(lián)免疫吸附檢測試劑盒
        ELK2040
        規(guī)格: 價格:
        48T ¥1960.00
        96T ¥2800.00

        Overview 文獻(xiàn)

        Product name: Rat IL19(Interleukin 19) ELISA Kit
        Reactivity: Rat
        Alternative Names: IL-19;MDA1; ZMDA1, IL-10C, NG.1; Melanoma Differentiation Associated Protein-Like Protein
        Assay Type: Sandwich
        Sensitivity: 6.8 pg/mL
        Standard: 1000 pg/mL
        Detection Range: 15.63-1000 pg/mL
        Sample Type: Serum, plasma and other biological fluids.
        Assay Length: 3.5h
        Research Area: Cytokine;Infection immunity;
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL19. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL19. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL19, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL19 in the samples is then determined by comparing the OD of the samples to the standard curve.

        標(biāo)準(zhǔn)曲線

        Concentration (pg/mL) OD Corrected OD
        1000.00 2.219 2.131
        500.00 1.636 1.548
        250.00 1.132 1.044
        125.00 0.872 0.784
        62.50 0.547 0.459
        31.25 0.300 0.212
        15.63 0.216 0.128
        0.00 0.088 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant IL19 and the recovery rates were calculated by comparing the measured value to the expected amount of IL19 in samples.
        Matrix Recovery range Average
        serum(n=5) 92-107% 99%
        EDTA plasma(n=5) 87-99% 93%
        Heparin plasma(n=5) 78-92% 85%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL19 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 86-94% 91-105% 87-99% 95-100%
        EDTA plasma(n=5) 85-90% 83-96% 95-102% 88-95%
        Heparin plasma(n=5) 83-103% 82-93% 86-95% 81-96%
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