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小鼠載脂蛋白E(APOE)酶聯(lián)免疫吸附檢測試劑盒

ELK2007

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Mouse APOE(Apolipoprotein E) ELISA Kit
Reactivity:	Mouse
Alternative Names:	Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
Assay Type:	Sandwich
Sensitivity:	6.7 ng/mL
Standard:	1000 ng/mL
Detection Range:	15.63-1000 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;Cardiovascular biology;Hepatology;
Uniprot ID:	P08226
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APOE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOE in the samples is then determined by comparing the OD of the samples to the standard curve.

Neurobiology of Disease

Prenatal stress aggravates age-dependent cognitive decline, insulin signaling dysfunction, and the pro-inflammatory response in the APPNL-F/NL-F mouse model of Alzheimer's disease

日期：2023-06-29 IF：6.1

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
1000.00	2.096	2.015
500.00	1.602	1.521
250.00	1.035	0.954
125.00	0.682	0.601
62.50	0.589	0.508
31.25	0.365	0.284
15.63	0.215	0.134
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant APOE and the recovery rates were calculated by comparing the measured value to the expected amount of APOE in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	92-105%	98%
Heparin plasma(n=5)	83-95%	89%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APOE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.