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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2000.00 | 1.948 | 1.862 |
1000.00 | 1.593 | 1.507 |
500.00 | 1.185 | 1.099 |
250.00 | 0.866 | 0.780 |
125.00 | 0.576 | 0.490 |
62.50 | 0.387 | 0.301 |
31.25 | 0.252 | 0.166 |
0.00 | 0.086 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 77-95% | 86% |
EDTA plasma(n=5) | 80-97% | 88% |
Heparin plasma(n=5) | 82-94% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 92-102% | 81-93% | 86-98% | 91-103% |
EDTA plasma(n=5) | 89-101% | 84-101% | 95-104% | 88-105% |
Heparin plasma(n=5) | 93-102% | 87-96% | 85-98% | 91-103% |
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