欧洲无码精品一二三区免费，无码AV在线一本无码，亚洲中文无码a∨在线观看，国内无码av不卡一区二区，人妻三级日本香港三级极97，亚洲高清无码三级片，国产日韩av综合在线观看，亚洲最大在线无码片

產(chǎn)品中心

人Toll樣受體9(TLR9)酶聯(lián)免疫吸附檢測試劑盒

ELK1948

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human TLR9(Toll Like Receptor 9) ELISA Kit
Reactivity:	Human
Alternative Names:	CD289
Assay Type:	Sandwich
Sensitivity:	0.062 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Immune molecule;
Uniprot ID:	Q9NR96
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TLR9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TLR9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TLR9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TLR9 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.054	1.959
5.00	1.527	1.432
2.50	1.128	1.033
1.25	0.901	0.806
0.63	0.532	0.437
0.32	0.305	0.210
0.16	0.196	0.101
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TLR9 and the recovery rates were calculated by comparing the measured value to the expected amount of TLR9 in samples.

Matrix	Recovery range	Average
serum(n=5)	88-101%	94%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	78-91%	84%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TLR9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.