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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 2.135 | 2.063 |
5.00 | 1.592 | 1.520 |
2.50 | 1.087 | 1.015 |
1.25 | 0.795 | 0.723 |
0.63 | 0.457 | 0.385 |
0.32 | 0.335 | 0.263 |
0.16 | 0.135 | 0.063 |
0.00 | 0.072 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 95-107% | 101% |
EDTA plasma(n=5) | 90-105% | 97% |
Heparin plasma(n=5) | 82-95% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 83-103% | 82-93% | 86-95% | 81-96% |
EDTA plasma(n=5) | 91-102% | 86-102% | 87-99% | 99-105% |
Heparin plasma(n=5) | 87-102% | 82-91% | 86-93% | 81-94% |
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