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人組織蛋白酶K(CTSK)酶聯(lián)免疫吸附檢測試劑盒

ELK1911

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Human CTSK(Cathepsin K) ELISA Kit
Reactivity:	Human
Alternative Names:	CTS-K; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD; Pycnodysostosis; Cathepsin O; Cathepsin X
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Infection immunity;Bone metabolism;
Uniprot ID:	P43235
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CTSK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTSK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CTSK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTSK in the samples is then determined by comparing the OD of the samples to the standard curve.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Inflammation-Driven Secretion Potential Is Upregulated in Osteoarthritic Fibroblast-Like Synoviocytes

日期：2022-10-05 IF：6.208
Int J Biol Sci

ETS1-mediated Regulation of SOAT1 Enhances the Malignant Phenotype of Oral Squamous Cell Carcinoma and Induces Tumor-associated Macrophages M2-like Polarization

日期：2024-06-11 IF：8.2

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.266	2.178
5.00	1.563	1.475
2.50	1.073	0.985
1.25	0.882	0.794
0.63	0.548	0.460
0.32	0.262	0.174
0.16	0.216	0.128
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CTSK and the recovery rates were calculated by comparing the measured value to the expected amount of CTSK in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	81-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTSK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.