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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
40.00 | 2.121 | 2.038 |
20.00 | 1.674 | 1.591 |
10.00 | 1.108 | 1.025 |
5.00 | 0.871 | 0.788 |
2.50 | 0.509 | 0.426 |
1.25 | 0.313 | 0.230 |
0.63 | 0.252 | 0.169 |
0.00 | 0.083 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 87-99% | 92% |
EDTA plasma(n=5) | 88-107% | 94% |
Heparin plasma(n=5) | 97-105% | 101% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 79-96% | 95-103% | 87-98% |
EDTA plasma(n=5) | 87-96% | 85-97% | 85-94% | 87-96% |
Heparin plasma(n=5) | 92-101% | 82-98% | 89-97% | 85-93% |
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