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人神經(jīng)絲蛋白3(NEF3)酶聯(lián)免疫吸附檢測試劑盒

ELK1883

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human NEF3(Neurofilament 3) ELISA Kit
Reactivity:	Human
Alternative Names:	NEFM; NF-M; NFM; Neurofilament,Medium Polypeptide; 160 kDa neurofilament protein; Neurofilament triplet M protein
Assay Type:	Sandwich
Sensitivity:	6.1 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cerebrospinal fluid and other biological fluids
Assay Length:	3.5h
Research Area:	Neuro science;Developmental science;
Uniprot ID:	P07197
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NEF3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NEF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEF3 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.266	2.184
500.00	1.647	1.565
250.00	1.213	1.131
125.00	0.872	0.790
62.50	0.567	0.485
31.25	0.365	0.283
15.63	0.186	0.104
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NEF3 and the recovery rates were calculated by comparing the measured value to the expected amount of NEF3 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-98%	91%
EDTA plasma(n=5)	91-99%	95%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEF3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.