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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2000.00 | 1.952 | 1.856 |
1000.00 | 1.579 | 1.483 |
500.00 | 1.182 | 1.086 |
250.00 | 0.828 | 0.732 |
125.00 | 0.529 | 0.433 |
62.50 | 0.323 | 0.227 |
31.25 | 0.179 | 0.083 |
0.00 | 0.096 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 95-104% | 98% |
EDTA plasma(n=5) | 82-90% | 86% |
Heparin plasma(n=5) | 84-92% | 87% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 82-96% | 87-103% | 95-102% |
EDTA plasma(n=5) | 89-97% | 85-94% | 87-101% | 85-96% |
Heparin plasma(n=5) | 85-94% | 87-101% | 82-95% | 82-93% |
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