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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2000.00 | 1.964 | 1.882 |
1000.00 | 1.616 | 1.534 |
500.00 | 1.043 | 0.961 |
250.00 | 0.869 | 0.787 |
125.00 | 0.548 | 0.466 |
62.50 | 0.335 | 0.253 |
31.25 | 0.198 | 0.116 |
0.00 | 0.082 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-92% | 86% |
EDTA plasma(n=5) | 90-105% | 97% |
Heparin plasma(n=5) | 88-102% | 95% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 79-92% | 89-102% | 87-98% | 80-92% |
EDTA plasma(n=5) | 98-106% | 86-99% | 78-95% | 89-97% |
Heparin plasma(n=5) | 85-94% | 87-96% | 92-101% | 82-98% |
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