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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
400.00 | 0.199 | |
200.00 | 0.396 | |
100.00 | 0.557 | |
50.00 | 0.861 | |
25.00 | 1.119 | |
12.50 | 1.565 | |
6.25 | 1.799 | |
0.00 | 2.115 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 86-99% | 92% |
EDTA plasma(n=5) | 79-93% | 86% |
Heparin plasma(n=5) | 90-105% | 97% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 95-106% | 87-96% | 82-90% | 92-101% |
EDTA plasma(n=5) | 85-92% | 95-103% | 97-105% | 93-102% |
Heparin plasma(n=5) | 88-97% | 97-105% | 81-93% | 89-102% |
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