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        人Salusin肽β(SALb)酶聯(lián)免疫吸附檢測試劑盒
        ELK1781
        規(guī)格: 價格:
        48T ¥1960.00
        96T ¥2800.00

        Overview

        Product name: Human SALb(Salusin Beta) ELISA Kit
        Reactivity: Human
        Alternative Names: Salusin Beta
        Assay Type: Competitive Inhibition
        Sensitivity: 1.75 pg/mL
        Standard: 400 pg/mL
        Detection Range: 6.25-400 pg/mL
        Sample Type: serum, plasma and other biological fluids
        Assay Length: 2h
        Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;
        Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SALb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SALb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SALb in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (pg/mL) OD Corrected OD
        400.00 0.199
        200.00 0.396
        100.00 0.557
        50.00 0.861
        25.00 1.119
        12.50 1.565
        6.25 1.799
        0.00 2.115

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant SALb and the recovery rates were calculated by comparing the measured value to the expected amount of SALb in samples.
        Matrix Recovery range Average
        serum(n=5) 86-99% 92%
        EDTA plasma(n=5) 79-93% 86%
        Heparin plasma(n=5) 90-105% 97%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SALb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 95-106% 87-96% 82-90% 92-101%
        EDTA plasma(n=5) 85-92% 95-103% 97-105% 93-102%
        Heparin plasma(n=5) 88-97% 97-105% 81-93% 89-102%
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