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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
12.00 | 1.946 | 1.859 |
6.00 | 1.528 | 1.441 |
3.00 | 1.194 | 1.107 |
1.50 | 0.806 | 0.719 |
0.75 | 0.559 | 0.472 |
0.38 | 0.324 | 0.237 |
0.19 | 0.258 | 0.171 |
0.00 | 0.087 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 79-97% | 88% |
EDTA plasma(n=5) | 81-97% | 89% |
Heparin plasma(n=5) | 85-97% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 83-97% | 84-99% | 95-102% | 88-103% |
EDTA plasma(n=5) | 86-95% | 91-106% | 87-100% | 95-101% |
Heparin plasma(n=5) | 95-102% | 83-97% | 88-96% | 96-103% |
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