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小鼠內(nèi)脂素(VF)酶聯(lián)免疫吸附檢測試劑盒

ELK1727

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Mouse VF(Visfatin) ELISA Kit
Reactivity:	Mouse
Alternative Names:	Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
Assay Type:	Sandwich
Sensitivity:	12.9 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;Cardiovascular biology;Hepatology;
Uniprot ID:	Q99KQ4
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VF in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.266	2.193
1000.00	1.527	1.454
500.00	1.109	1.036
250.00	0.821	0.748
125.00	0.516	0.443
62.50	0.312	0.239
31.25	0.186	0.113
0.00	0.073	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VF and the recovery rates were calculated by comparing the measured value to the expected amount of VF in samples.

Matrix	Recovery range	Average
serum(n=5)	92-106%	99%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	88-103%	95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.