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        大鼠胰蛋白酶原激活肽(TAP)酶聯(lián)免疫吸附檢測試劑盒
        ELK1723
        規(guī)格: 價格:
        48T ¥1960.00
        96T ¥2800.00

        Overview

        Product name: Rat TAP(Trypsinogen Activation Peptide) ELISA Kit
        Reactivity: Rat
        Alternative Names: Trypsinogen Activation Peptide
        Assay Type: Competitive Inhibition
        Sensitivity: 50.3 pg/mL
        Standard: 10000 pg/mL
        Detection Range: 156.25-10000 pg/mL
        Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
        Assay Length: 2h
        Research Area: Infection immunity;Immune molecule;Hepatology;
        Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TAP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TAP in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (pg/mL) OD Corrected OD
        10000.00 0.183
        5000.00 0.310
        2500.00 0.596
        1250.00 0.895
        625.00 1.169
        312.50 1.585
        156.25 1.697
        0.00 2.256

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant TAP and the recovery rates were calculated by comparing the measured value to the expected amount of TAP in samples.
        Matrix Recovery range Average
        serum(n=5) 80-95% 87%
        EDTA plasma(n=5) 83-97% 90%
        Heparin plasma(n=5) 78-92% 85%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 79-94% 87-96% 78-93% 82-90%
        EDTA plasma(n=5) 95-102% 89-96% 87-98% 85-93%
        Heparin plasma(n=5) 92-103% 93-105% 88-101% 97-106%
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