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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
10000.00 | 0.183 | |
5000.00 | 0.310 | |
2500.00 | 0.596 | |
1250.00 | 0.895 | |
625.00 | 1.169 | |
312.50 | 1.585 | |
156.25 | 1.697 | |
0.00 | 2.256 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 83-97% | 90% |
Heparin plasma(n=5) | 78-92% | 85% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 79-94% | 87-96% | 78-93% | 82-90% |
EDTA plasma(n=5) | 95-102% | 89-96% | 87-98% | 85-93% |
Heparin plasma(n=5) | 92-103% | 93-105% | 88-101% | 97-106% |
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