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小鼠補(bǔ)體因子H(CFH)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1722

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Mouse CFH(Complement Factor H) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
Assay Type:	Sandwich
Sensitivity:	3.7 ng/mL
Standard:	500 ng/mL
Detection Range:	7.82-500 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;Infection immunity;Immune molecule;
Uniprot ID:	P06909
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CFH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CFH in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
500.00	1.948	1.853
250.00	1.613	1.518
125.00	1.073	0.978
62.50	0.883	0.788
31.25	0.521	0.426
15.63	0.317	0.222
7.82	0.161	0.066
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CFH and the recovery rates were calculated by comparing the measured value to the expected amount of CFH in samples.

Matrix	Recovery range	Average
serum(n=5)	80-96%	88%
EDTA plasma(n=5)	80-92%	86%
Heparin plasma(n=5)	78-95%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CFH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.