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人谷胱甘肽S轉(zhuǎn)移酶α5(GSTa5)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1716

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Human GSTa5(Glutathione S Transferase Alpha 5) ELISA Kit
Reactivity:	Human
Alternative Names:	GST-a5; Glutathione S-Transferase A5; GST class-alpha member 5
Assay Type:	Sandwich
Sensitivity:	0.235 ng/mL
Standard:	40 ng/mL
Detection Range:	0.63-40 ng/mL
Sample Type:	Serum, plasma and other biological fluids.
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Tumor immunity;Hepatology;
Uniprot ID:	Q7RTV2
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSTa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSTa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSTa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSTa5 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
40.00	2.126	2.037
20.00	1.536	1.447
10.00	1.196	1.107
5.00	0.852	0.763
2.50	0.554	0.465
1.25	0.372	0.283
0.63	0.175	0.086
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GSTa5 and the recovery rates were calculated by comparing the measured value to the expected amount of GSTa5 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	86-99%	92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GSTa5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.