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小鼠丙酮酸脫氫酶α(PDHa)酶聯(lián)免疫吸附檢測試劑盒

ELK1710

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Mouse PDHa(Pyruvate Dehydrogenase Alpha) ELISA Kit
Reactivity:	Mouse
Alternative Names:	PDHCE1A; PHE1A; PHE1-A; PDHA1; PDH-A; Pyruvate Dehydrogenase(lipoamide)Alpha 1; Pyruvate Dehydrogenase E1 Component Subunit Alpha,Aomatic Form,Mitochondrial
Assay Type:	Sandwich
Sensitivity:	34 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Metabolic pathway;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDHa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDHa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDHa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDHa in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.131	2.036
2500.00	1.579	1.484
1250.00	1.194	1.099
625.00	0.896	0.801
312.50	0.453	0.358
156.25	0.313	0.218
78.13	0.230	0.135
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PDHa and the recovery rates were calculated by comparing the measured value to the expected amount of PDHa in samples.

Matrix	Recovery range	Average
serum(n=5)	78-96%	87%
EDTA plasma(n=5)	78-92%	85%
Heparin plasma(n=5)	80-95%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDHa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.