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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2000.00 | 2.045 | 1.947 |
1000.00 | 1.657 | 1.559 |
500.00 | 1.035 | 0.937 |
250.00 | 0.883 | 0.785 |
125.00 | 0.496 | 0.398 |
62.50 | 0.327 | 0.229 |
31.25 | 0.175 | 0.077 |
0.00 | 0.098 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 83-99% | 95% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 85-97% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-95% | 93-103% | 87-98% | 85-96% |
EDTA plasma(n=5) | 91-99% | 83-89% | 88-104% | 97-103% |
Heparin plasma(n=5) | 85-95% | 79-96% | 89-101% | 85-97% |
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