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人解整合素金屬蛋白酶8(ADAM8)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1695

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Human ADAM8(A Disintegrin And Metalloprotease 8) ELISA Kit
Reactivity:	Human
Alternative Names:	CD156-A; MS2; CD156a; Disintegrin and metalloproteinase domain-containing protein 8
Assay Type:	Sandwich
Sensitivity:	2.37 pg/mL
Standard:	400 pg/mL
Detection Range:	6.25-400 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Tumor immunity;Immune molecule;Neuro science;
Uniprot ID:	P78325
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADAM8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADAM8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADAM8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADAM8 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
400.00	2.266	2.180
200.00	1.593	1.507
100.00	1.118	1.032
50.00	0.852	0.766
25.00	0.553	0.467
12.50	0.385	0.299
6.25	0.187	0.101
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ADAM8 and the recovery rates were calculated by comparing the measured value to the expected amount of ADAM8 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	80-95%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADAM8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.