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大鼠核因子κB亞基p65(NFκB-p65)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1693

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Rat NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit
Reactivity:	Rat
Alternative Names:	NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
Assay Type:	Sandwich
Sensitivity:	0.058 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Apoptosis;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

Inflammopharmacology

Combinatorial intervention with dental pulp stem cells and sulfasalazine in a rat model of ulcerative colitis

日期：2024-04-28 IF：4.6

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	1.978	1.891
5.00	1.536	1.449
2.50	1.184	1.097
1.25	0.841	0.754
0.63	0.554	0.467
0.32	0.381	0.294
0.16	0.190	0.103
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NFκB-p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NFκB-p65 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-97%	90%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	85-95%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NFκB-p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.