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人血小板膜糖蛋白Ⅱb/Ⅲa(GPIIb/IIIa)酶聯(lián)免疫吸附檢測試劑盒

ELK1688

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human GPIIb/IIIa(Glycoprotein IIb/IIIa) ELISA Kit
Reactivity:	Human
Alternative Names:	GP-IIb/IIIa
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	CD & Adhesion molecule;Metabolic pathway;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPIIb/IIIa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPIIb/IIIa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPIIb/IIIa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPIIb/IIIa in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	1.966	1.886
5.00	1.518	1.438
2.50	1.052	0.972
1.25	0.861	0.781
0.63	0.533	0.453
0.32	0.325	0.245
0.16	0.176	0.096
0.00	0.080	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GPIIb/IIIa and the recovery rates were calculated by comparing the measured value to the expected amount of GPIIb/IIIa in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	84-102%	93%
Heparin plasma(n=5)	87-95%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPIIb/IIIa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.